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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: COL4A1 promotes the growth and metastasis of hepatocellular carcinoma cells by activating FAK-Src signaling

Fig. 4

RUNX1 activates the transcription of COL4A1 in HCC. a Correlation between mRNA levels of COL4A1 and RUNX1. Linear regression analysis showed the positive correlation between mRNA levels of RUNX1 and COL4A1 from HCC samples in TCGA database (r = 0.5800, P < 0.0001). b mRNA level of RUNX1 was upregulated in cancerous tissues (C, n = 374) compared with noncancerous liver tissues (N, n = 50) from TCGA datasets. c&d RUNX1 regulates COL4A1 expression. Protein levels of COL4A1 and RUNX1 were detected by western blot analysis in indicated HCC cell lines after transfected with either overexpression vector (HA-RUNX1) (c) or si-RUNX1 (d). e&f RUNX1 activates transcription of COL4A1 by dual luciferase reporter assay. Overexpression of RUNX1 activated the transcription of COL4A1 (e) and knockdown of RUNX1 inhibited the transcription of COL4A1 (f). Student t test, *P < 0.05, **P < 0.01, ***P < 0.001

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