Fig. 6

FAK or Src inhibitor selectively inhibits the cell viability of HCC cells with high expression of COL4A1. a&b Cell viability was tested after treatment with inhibitors. HCC cells were treated with Defactinib (FAK inhibitor) or Saracatinib (Src inhibitor) at the indicated concentrations for 48 h. HCC cells with high expression level of COL4A1 (a) were sensitive to Defactinib or Saracatinib treatment, but HCC cells with low expression level of COL4A1 (b) were resistance to Defactinib or Saracatinib treatment. c&d Knockdown of COL4A1 reduced the sensitive to Defactinib or Saracatinib treatment in SMMC7721 (c) and SK-Hep1 (d). Indicated cells were treated with Defactinib (FAK inhibitor) or Saracatinib (Src inhibitor) at the indicated concentrations for 48 h. e&f Overexpression of COL4A1 increased the sensitive to Defactinib or Saracatinib treatment in HepG2 (e) and PLC/PRF/5 (f). Indicated cells were treated with Defactinib (FAK inhibitor) or Saracatinib (Src inhibitor) at the indicated concentrations for 48 h. Cell viability was analyzed by crystal violet staining assay (Left) and CCK8 assay (Right), respectively. Data are presented as means ± standard deviation. Student t test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant