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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Genipin suppression of growth and metastasis in hepatocellular carcinoma through blocking activation of STAT-3

Fig. 3

Genipin inhibits HCC cell proliferation and angiogenesis. (a) MHCC97L, HepG2, or normal haptic cells (LO2) were treated with different dosages of genipin for 24 h. Cell viability was detected by MTS assay. (b) STAT-3 vector was transfected into MHCC97L cells. Cell viability was detected by MTS assay, and protein expression of STAT-3 target genes was examined by western blotting. (c) HCC cells were pre-treated with different concentrations of genipin for 24 h; cell apoptosis was analyzed by flow cytometry using Annexin V/7AAD commercial kit. Expressions of PARP and cleaved PARP were examined by western blot assay. (d) Colony formation assays were used to detect HCC cell proliferation ability. MHCC97L and HepG2 cells were incubated with 20-μM genipin for 2 weeks. Results were shown in the histogram from three independent experiments. (e) Angiogenesis was detected by tube formation assay. 1 × 105 HUVECs were cultured on Matrigel. Cells were incubated with genipin for 10 h; representative images of tube formation were observed under an inverted microscope (Leica, Germany) and the relative tube numbers were analyzed. The data presented as mean ± SD. *P < 0.05 and **P < 0.01

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