Fig. 1

IMP3 expression was elevated in prostate cancer tissues and cells. a-b RT-PCR and western blotting assays were performed to examine the mRNA and protein expression levels of IMP3 in 20 paired prostate cancer tissues and normal tissues, the representative blots were shown in (c). IHC technology was carried out to test IMP3 protein levels in prostate cancer tissues and the matched normal tissues with anti-IMP3 antibody, and the representative images of each group were shown in (d-e). RT-PCR and western blotting assays were performed to examine the mRNA and protein expression levels of IMP3 in RWPE-1, LNCap, PC3 and DU145 cell lines. (^**p < 0.01, ^***p < 0.001, compared with normal/RWPE-1 group)