Fig. 2

Overexpression of IMP3 promoted prostate cancer cell proliferation and repressed cell apoptosis. a-b The mRNA and protein expression levels of IMP3 in PC3, LNCap and DU145 cells of si-NC, si-IMP3, OE-NC and OE-IMP3 groups were determined by using RT-PCR and western blotting assays, respectively. c-e Cell proliferation ability was determined by CCK-8 assay in different groups of PC3, LNCap and DU145 cells, including si-IMP3, si-NC, OE-IMP3 or OE-NC groups. f Cell clone formation ability was tested by clone formation assay after PC3, LNCap and DU145 cells were transfected with si-IMP3, si-NC, OE-IMP3 or OE-NC. g-h The apoptosis rates of PC3, LNCap and DU145 cells in si-IMP3, si-NC, OE-IMP3 or OE-NC groups were tested by flow cytometry assay (Cells in Q2 and Q3 were considered as the apoptotic cells). (^*p < 0.05, ^***p < 0.001, si-IMP3 group compared with si-NC group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, OE-IMP3 group compared with OE-NC group)