Fig. 4

IMP3 positively modulated SMURF1 expression in DU145 and LNCap cells. a-b. Following cell transfection/infection with si-IMP3, si-NC, OE-IMP3 or OE-NC, total RNA was isolated from cells and western blotting assay was performed to detect the protein expression levels of IMP3, CDC4, RCHY1, MDM2, SKP2, UBE3A and SMURF1. c-d. The expression of SMURF1 mRNA was determined by RT-PCR in different groups of DU145 and LNCap cells, including si-IMP3, si-NC, OE-IMP3 and OE-NC groups. (^*p < 0.05, ^***p < 0.001, si-IMP3 group compared with si-NC group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, OE-IMP3 group compared with OE-NC group)