Fig. 3

LINC00514 promoted tumor growth and pulmonary metastasis in vivo. (a-b) The images of subcutaneous tumors were obtained on Day 28. (c-f) The tumor volumes and weights of sh-LINC00514 group compared with NC group and LINC00514 overexpression group compared with empty group were quantified. Tumor volumes were analyzed by ANOVA. (g-h) qRT-PCR was used to assess the transfection efficiency. (i-j) The image of pulmonary metastasis was photographed at the endpoint. (k-l) Pulmonary metastasis of LINC00514 silencing and LINC00514 overexpression compared with their control groups were evaluated. *p<.05, **p<.01, **p<.001. The mean±SD was used to represent the final results of experiments repeated at least three times. PC, pancreatic cancer; qRT-PCR, quantitative real-time polymerase chain reaction; SD, standard deviation; NC, negative control; ANOVA, analysis of variance