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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Long noncoding RNA LINC00514 accelerates pancreatic cancer progression by acting as a ceRNA of miR-28-5p to upregulate Rap1b expression

Fig. 5

Rap1b was a downstream target of miR-28-5p in PC. (a) The sequence of WT-Rap1b, Mut-Rab1b and miR-28-5p were conducted. (e-f) Luciferase reporter assay and RIP assay were performed to determine the association between miR-28-5p and Rap1b. (f-g) QRT-PCR and western blot were used to detect Rap1b expression in cells of LINC00514 silencing and cells of co-transferring miR-28-5p and LINC00514 shRNA at transcription and translation level. (h) Relative Rap1b expression in tumor tissue and normal tissue were detected by qRT-PCR. (i-g) The correlation between Rap1b and LINC00514 as well as the correlation between Rap1b and miR-28-5p were analyzed by Spearman’s rank correlation test. (k-l) The Rab1b expression levels under LINC00514 silencing and LINC00514 overexpression were evaluated in vivo. (m-n) CCK-8 assay was performed to detect proliferation of cells transfected with miR-28-5p inhibitor and cells co-transfected with miR-28-5p inhibitor and Rap1b shRNA. (O-P) Transwell migration and invasion assay were carried out to detect cell migration and invasion abilities. *p<.05, **p<.01, **p<.001. All experiments were repeated at least for three times and mean±SD was used to represent the final result. PC, pancreatic cancer; qRT-PCR, quantitative real-time polymerase chain reaction; RIP, RNA immunoprecipitation; SD, standard deviation

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