Fig. 5

Effect of ZFPM2-AS1 on the biological function of miR-139 in HCC cells. a CCK-8 assay of Huh7 and HCCLM3 cells transfected with miR-NC, miR-139, miR-139 + pcDNA-NC and miR-139 + pcDNA/ZFPM2-AS1 at 0 h, 24 h, 48 h and 72 h to measure cell viability. b Colony formation assay to detect the colony formation ability of HCCLM3 cells transfected with miR-NC, miR-139 and miR-139 + pcDNA/ZFPM2-AS1.The right side is the number of cell clones. c Flow cytometry was used to detect the cell cycle distribution of miR-NC-, miR-139- and miR-139 + pcDNA/ZFPM2-AS1-transfected HCCLM3 cells, and the right side is the cell cycle distribution. d-e Transwell chamber assay was used to detect the migration (d) and invasion (e) ability of miR-NC-, miR-139- and miR-139 + pcDNA/ZFPM2-AS1-transfected HCCLM3 cells. The right side is a statistical graph of the migrating cell count and the invasive cell count, respectively. f Huh7 cell xenograft tumor growth curve. miR-139- and miR-139 + pcDNA/4 ZFPM2-AS1-transfected Huh7 cells were injected into the axilla of nude mice for 6 weeks, and the nude mice were dissected for tumor tissue. Statistical data are expressed as the mean ± standard deviation, **P < 0.01 (vs miR-NC), ^##P < 0.01, ^###P < 0.001 (vs miR-139)