Fig. 5

Effect of HG, RCS, AGE and FL-926-16 on human PDA cell proliferation, ERK 1/2 phosphorylation status and YAP activation. MIA PaCa-2 (a) and Panc-1 (b) cell proliferation after 48 h incubation with high glucose (HG, 25 mM) vs normal glucose (NG, 5 mM), with or without the carbonyl trapping agent FL-926-16 (FL, 20 mM). PDA cell (MIA PaCa-2, c) proliferation after 48 h incubation with the RCS glyoxal (GO) and methylglyoxal (MGO), 200 μM each, or the preformed AGE CML (100 μg/mL), with or without 20 mM FL, and relative representative bright-field images (original magnification: 100X, scale bar: 200 μm); n = 5 wells in duplicate per condition. Western blot analysis for phosphorylated and total ERK 1/2 in lysates (d) and YAP in nuclear extracts (e) from MIA PaCa-2 cells exposed to MGO (200 μM) or CML (100 μg/mL) for 48 h, with or without FL (20 mM), and relative band densitometry analysis from three separate experiments. Each dot in (a), (b) and (c), represents one well and bars represent mean ± SEM. Each dot in (d) and (e) represents a single experiment and bars represent mean ± SEM. Post hoc multiple comparison: ***P < 0.001 or *P < 0.05 vs Ctr; †††P < 0.001, ††P < 0.01 or †P < 0.05 vs untreated