Fig. 6

Inhibiting p38 phosphorylation reduced autophagy in vitro. a-b Effects of p38 de-phosphorylation on LC3B expression in U87 cells according to immunofluorescence. Scale bars, 100 μm. **P < 0.01. c-d Cells were prepared for transmission electron microscopy analysis. The number of autophagic vacuoles was analysed and the red arrows indicate autophagic vacuoles. **P < 0.01. e Effects of p38 de-phosphorylation on the levels of autophagy-related proteins in U87 cells