Fig. 3

LINC00669 physically binds to SOCS1. a, Silver staining of the proteins pulled down from LINC00669 sense and antisense RNAs incubated CNE-2 cell lysates. AS, antisense; S, sense. b, Western blotting confirming the interaction between LINC00669 and SOCS1 in NPC cells. c, qPCR analysis of LINC00669 enrichment in SOCS1-immunoprecipitated RNAs in control and LINC00669-depleted NPC cell lysate. Antibody against normal human IgG was included as a RIP negative control. d and e, interaction profile (d) and interaction matrix (e) demonstrating catRAPID fragments-based prediction of interaction between LINC00669 and SOCS1. f, Schematic diagram showing construction of the truncated LINC00669. g, Western blotting showing only the truncated LINC00669 constructs encompassing 190–283 nt domain were able to pulldown SOCS1