Fig. 4
SOCS1 plays as a tumor suppressor in NPC cells by regulating STAT1 protein. a, qPCR analysis confirming ectopic expression (SOCS1) and knockdown of SOCS1 (siSOCS1) in NPC cells. *** p < 0.001 versus negative control (NC) group. b, Western blotting showing the effects of SOCS1 in the expression of the JAK/STAT signaling pathway component proteins. STAT1 was decreased when overexpressing SOCS1, while was increased when silencing SOCS1. c, MTT assay showing the inhibitory effect of SOCS1 on the time-dependent proliferation in NPC cells, which could be partially rescued upon the simultaneous depletion of STAT1. NC, negative control; siSOCS1, siRNA-mediated silencing of SOCS1; siSTAT1, siRNA-mediated silencing of STAT1. d, Transwell assay showing the invasion-suppressive effect of SOCS1 in NPC cells, which could be reversed upon simultaneous depletion of STAT1. * p < 0.05, ** p < 0.01, *** p < 0.001 versus NC group. e, Wound-healing assay showing the migration-inhibiting effect of SOCS1 in NPC cells, which could be reversed by simultaneous depletion of STAT1. * p < 0.05, ** p < 0.01, *** p < 0.001 versus NC group. f, Representative flow cytometry plots showing the pro-apoptotic effect of SOCS1 in NPC cells. * p < 0.05, ** p < 0.01, *** p < 0.001 versus NC group