Table 2 Antibody list
Primary antibodies | MW (kDa) | Dilution | Company / Catalog | Secondary antibodies | Dilution |
|---|---|---|---|---|---|
SOCS1 | ≈38 | 1:500 | Santa Cruz, sc-518,028 | Goat Anti Rabbit IgG/HRP | 1:4000 |
JAK2 | ≈120 | 1:500 | omnimabs,OM206683 | Goat Anti Rabbit IgG/HRP | 1:4000 |
JAK3 | ≈125 | 1:1000 | omnimabs,OM260121 | Goat Anti Mouse IgG/HRP | 1:4000 |
STAT1 | ≈87 | 1:1000 | Abcam,ab234400 | Goat Anti Rabbit IgG/HRP | 1:4000 |
STAT3 | ≈88 | 1:2000 | Abcam,ab119352 | Goat Anti Mouse IgG/HRP | 1:4000 |
p-STAT1(Y701) | ≈87 | 1:1000 | Abcam,ab29045 | Goat Anti Mouse IgG/HRP | 1:4000 |
UB | ≈8.4 | 1:200 | Ptgcn,10,201–2-AP | Goat Anti Rabbit IgG/HRP | 1:4000 |
HIS | Â | 1:500 | Abcam,ab18184 | Goat Anti Mouse IgG/HRP | 1:4000 |
GFP | Â | 1:1000 | Abcam,ab290 | Goat Anti Rabbit IgG/HRP | 1:4000 |
FLAG | Â | 1:1000 | Abcam,ab205606 | Goat Anti Rabbit IgG/HRP | 1:4000 |
β-actin | 42 | 1:2000 | Ptgcn, 66,009–1-Ig | Goat Anti Mouse IgG/HRP | 1:4000 |