Fig. 1

Highly expressed linc00514 promotes breast cancer malignancy. a. Top 100 dysregulated lncRNAs whose P < 0.05, log₂FC > 2, or logFC < 0.5 were compared in the GSE60689 dataset and the GSE112848 dataset from the GEO database. b. The relative expression of linc00514 in tumor tissues (BRC) and in paracancer tissues (ANT) from 46 breast cancer patients was detected using qRT-PCR. c. The relative expression of linc00514 in human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and MCF-7) and in the normal breast epithelial cell line (MCF-10A) was detected using qRT-PCR. **P < 0.01 vs MCF-10A. d-f. Human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and MCF-7) were transiently transfected with linc00514 siRNAs (Si-linc00514) or linc00514 plasmids (pcDNA-linc00514) for 48 h. Cell viability was detected using MTT assay (d). Cell invasion was detected using Transwell assay (e, f). Scale bar = 200 μm. g-h. Female Balb/c nude mice were subcutaneously injected with the MDA-MB-468 cells (5 × 10⁶ cells) which were stably transfected with linc00514-overexpressing plasmids (linc00514-OVE) or the control plasmids (n = 5 in each group). The tumor volumes were detected every week (g). After 7 weeks, the Ki67 expression and the Vimentin expression in tumor tissues were detected using immunohistochemistry (h). Scale bar = 80 μm. i. Female Balb/c nude mice were intravenously injected with the MDA-MB-231/MCF-7 cells (1.5 × 10⁶ cells) which were stably transfected with linc00514-OVE (n = 5 in each group). The pulmonary metastatic tumor tissues were observed using HE staining. Scale Bar = 160 μm. Three independent experiments. *P < 0.05, **P < 0.01 vs control (Si-ctrl). ##P < 0.01 vs control (pcDNA)