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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Linc00514 promotes breast cancer metastasis and M2 polarization of tumor-associated macrophages via Jagged1-mediated notch signaling pathway

Fig. 3

Linc00514 positively regulates the protein expression of pSTAT3 and Jagged1. a. Female Balb/c nude mice were subcutaneously injected with the MDA-MB-468 cells (5 × 106 cells) which were stably transfected with linc00514-overexpressing plasmids (linc00514-OVE) or the control plasmids (n = 5 in each group). After 7 weeks, the expression of pSTAT3 and Jagged1 in tumor tissues was detected using immunohistochemistry. Scale bar = 200 μm. b. Human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and MCF-7) were transiently transfected with linc00514 siRNAs (Si-linc00514) or linc00514 plasmids (pcDNA-linc00514) for 48 h. The expression of pSTAT3, STAT3, and Jagged1 was detected using western blot analysis. c-e. Human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and MCF-7) were transiently transfected with STAT3 siRNAs (Si-STAT3) or JAG1 siRNAs (Si-JAG1) for 48 h. Cell invasion was detected using MTT assay (c). Scale bar = 200 μm. The protein level of pSTAT3 and Jagged1 was detected using western blot analysis (d). The relative mRNA expression of JAG1 and HES1 was detected using qRT-PCR (e). f. The 293 T cells were transfected with human or mouse STAT3 vectors or empty vectors as well as JAG1 promoter plasmids. The relative luciferase activity was detected using luciferase reporter assay. Three independent experiments. *P < 0.05, ** P < 0.01 vs control (Si-ctrl). #P < 0.05, ##P < 0.01 vs control (Si-ctrl or pcDNA)

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