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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Linc00514 promotes breast cancer metastasis and M2 polarization of tumor-associated macrophages via Jagged1-mediated notch signaling pathway

Fig. 5

Overexpression of linc00514 promotes proliferation and invasion of breast cancer cells and M2 polarization of macrophages via regulating STAT3. Human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and MCF-7) which were stably transfected with linc00514 plasmids (linc00514-OVE) were then transfected with STAT3 siRNAs (Si-STAT3) for 48 h. a. The cell proliferation was detected using EdU labeling detection. Scale Bar =100 μm. b. The cell invasion was detected using Transwell assay. Scale bar = 200 μm. c-d. After co-culturing breast cancer cells with PMA-induced human monocyte THP-1 cells for 48 h using Transwell assay, we detected the relative percentage of CD206+ and CD163+ in THP-1 derived macrophages using flow cytometry (c). The relative mRNA expression of JAG1 and HES1 was detected using qRT-PCR (d). Three independent experiments. *P < 0.05, **P < 0.01 vs control (linc00514-OVE)

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