Fig. 6

Linc00514 increases phosphorylation of STAT3. a-b. The distribution and localization of linc00514 in human breast cancer cell lines (MDA-MB-231, MDA-MB-468, and MCF-7) were detected using cytosol/nucleus qRT-PCR (a) and FISH (b). c. Human breast cancer cell lines (MDA-MB-231 and MCF-7) were transfected with the increasing concentration of linc00514 vectors (from 0 to 5 μg). The protein level of pSTAT3 and STAT3 was detected using western blot analysis. d. The siRNA-mediated JAK2 knockdown reduced the pSTAT3 protein level which was increased by linc00514 overexpression. e-f. The RNA pull-down experiment (e) and the RIP experiment (f) confirmed that linc00514 could bind with STAT3 and JAK2. g. Human breast cancer cell lines (MDA-MB-231 and MCF-7) were transfected with the increasing concentration of linc00514 vectors (from 0 to 5 μg). The levels of STAT3 and JAK2 pulled down by linc00514 probes were detected using the RNA pull-down experiment. h. Schema depicting the mechanisms of linc00514 in breast cancer growth and metastasis. Three independent experiments. **P < 0.01 vs control (Anti-IgG)