Linc00514 promotes breast cancer metastasis and M2 polarization of tumor-associated macrophages via Jagged1-mediated notch signaling pathway

Table 2 The primers, small interfering RNA sequences, and probes used in this study

qRT-PCR Primers (from 5′ to 3′)
Linc00514	F: GTGTGCCTGCCCTTTCCTGTG
	R: TCCCATCCCATTCCCATCACCAG
CD206	F: TTCAGTGGACCATCGAGGAAGAGG
	R: ATGGCAACACACCCTGGCTTTC
CD163	F: GCCACAACAGGTCGCTCATCC
	R: GCAAGCCGCTGTCTCTGTCTTC
Jag1	F: TGTGGCTTGGATCTGTTGCTTGG
	R: ACGTTGTTGGTGGTGTTGTCCTC
Hes1	F: GGCTGGAGAGGCGGCTAAGG
	R: TGCTGGTGTAGACGGGGATGAC
GAPDH	F: CAATGACCCCTTCATTGACC
	R: GACAAGCTTCCCGTTCTCAG
Si-RNA sequences
Si-control	UUCUCCGAACGUGUCACGUCU
Si-linc00514	GGACCACACUGGGAUUCUAGA
Si-STAT3	GAGUCAAGGAGACAUGCAAGA
Si-jag1	GGGUCAGAAUUGUGACAUAAA
Si-jak2	GAUGGAUAUUAUAGAUUAACU
Agarose gel electrophoresis primers
Linc00514	F: CAGGACCTTCGCAGGTGCTG
	R: CTTCTGGGGTTTTCCACCCCC
RNA Pull down Probe
CATCCCTTCCTCTCTGCTCAGGACCAGGGCCAGGCCCCTACATCAGTAACCCCCAACCCCTAAGACCTCCATGCTCCCAGAACTCCAGGGCCTTACCTGCCTGGGTTCCAGCTCCCAGGAAGGCCCCGACTCCCCACCCATTCCTATTCCCAAATCCCAGGGGACAGAGCCAAAGAG
FISH Probe
GCCATGTGGAGTGCGGGTCCGTGGTCCAAACACCACCCAGGGCCACTGTGCTCCACACCCTGACAGTTCACTAAGGGCGCATGCCCAGGAAGCAGCTCAGAGGCCTCCACAGGAGTCCCCACCCACAGGAATGGCATTGGCAGGCCACAGAGGGACGAGTGCCTGGGGAGGGTAACACAGACCGAGAGGGGGACAGAGAGGGACAGGACATGGTGAGTGTGGCATGGAGGTGGCACACGGGCGGGGTCCTCCCTTCATCCGCTTCTGAGGTTCCCTCTTCCCCAGGCCTGGGGAGAGCTAGAAAGAGGCTGACATTGACCCTAAGAGCTCCTCCCCTAAGAATGAGGGTTGGGACCCTAAACCCTCCTGCCCGCCCCTCCCTGCGGCCCCTGCCCAGCCACCCTCACCTG

Abbreviation: si-RNA Small interfering RNA; FISH Fluorescence in situ hybridization

ISSN: 1756-9966