Fig. 1

Fusobacterium nucleatum contributes to CRC. Male BALB/C nude mice were subcutaneously injected with HCT16 and HT29 cells treated with PBS, Escherichia coli, or Fusobacterium nucleatum to establish xenograft tumor animal models. a Tumor volume in HT29 and HCT116 cells. b Weight of tumors in nude mice formed by cells with different treatments. c Representative micrographs showing Ki-67 immunohistochemistry in xenograft tumor tissues (400 ×). d Number of tumors and tumor load in APC (Min/+) mice (n = 10 per group). e Representative micrographs showing PCNA (400 ×) and γH2AX (400 ×) immunohistochemistry. *p < 0.05 vs. PBS or Escherichia coli. Data are expressed as mean ± s.d. Data from multiple groups were compared using one-way analysis of variance (ANOVA) and Tukey’s post hoc test. Data comparison at different time points was performed using repeated measures ANOVA, followed by Bonferroni post hoc test. Non-parametric Mann-Whitney U test was used for comparison of data that were not normally distributed