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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: FadA promotes DNA damage and progression of Fusobacterium nucleatum-induced colorectal cancer through up-regulation of chk2

Fig. 3

Fusobacterium nucleatum induces DNA damage in CRC cells via FadA. HCT16 and HT29 cells were treated with WT or FadA−/− Fusobacterium nucleatum (MOI: 1000). a The number of cells counted by hemocytometry at at hour 6, 24 and 48 after treatment to detect cell proliferation. b Representative micrographs showing Comet analysis. c Representative micrographs (400 ×) depicting the γH2AX immunofluorescence assay. d γH2AX formation as determined by flow cytometry. e The number of cells in different cell cycle phases *p < 0.05 vs. WT Fusobacterium nucleatum. Data are expressed as mean ± s.d. Data from two groups were compared using the unpaired t test. Data comparison at different time points was performed using repeated measures ANOVA, followed by Bonferroni post hoc test. Each experiment was repeated three times

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