Fig. 3

Effects of EIF3H on ESCC cell motility and tumor metastasis in vitro and in vivo. EIF3H was stably overexpressed (a and b) or knocked down (c and d) in KYSE150 and KYSE510 cells, in which migration and invasion assays were performed. The migratory and invasive cells were counted. Data are obtained from three independent experiments. Scale bars, 500 μm. e EIF3H knockdown suppressed lung metastasis in vivo in mice. KYSE150 cells with or without knockdown of EIF3H were injected into the tail vein of SCID/Beige mice (n = 6) and the mice lung was harvested, weighed and stained 1 month later. HE staining was performed to analyze the nodules inside the lung. The lung weight was measured after the experiment. Representative images, representative HE staining (left panel) and quantitative results of lung weight (right panel) are shown. Scale bars, 1 mm. *P < 0.05; **P < 0.01; ***P < 0.001