Fig. 4

EIF3H interacts with Snail. a Vector or EIF3H-Flag plasmid was transfected in HEK293T cells for 24 h. After a 4 h treatment of 10 μM MG132, cell lysate was immunoprecipitated with anti-FLAG M2 beads, and the proteins interact with EIF3H were analyzed by mass spectrometry. Silver staining image was shown in the left panel. Representative proteins detected in mass spectrometry were shown in the right panel. b and c EIF3H-Flag was co-expressed with the Snail plasmid in HEK293T cells (b). Snail-Flag was co-transfected with the EIF3H plasmid in HEK293T cells (c). The co-IP assay was performed as described in (a). d Endogenous Snail was captured by anti-Snail antibody from KYSE510 cells, and the endogenous EIF3H and Snail were examined by immunoblotting. e Endogenous EIF3H and Snail in KYSE510 cells was detected by immunofluorescence staining. Scale bars, 30 μm. f The Flag-tagged empty vector, wild-type Snail or mutants plasmids were co-expressed with EIF3H plasmid. Extracts were subjected to the co-IP assay