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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: EIF3H promotes aggressiveness of esophageal squamous cell carcinoma by modulating Snail stability

Fig. 6

EIF3H are required for Snail-mediated EMT in ESCC cells. a EIF3H was stably expressed in KYSE150 and KYSE510 cells. Snail, EIF3H, E-cadherin, N-cadherin and Vimentin protein levels were analyzed by Immunoblotting in KYSE150-EIF3H (left panel) and KYSE510-EIF3H overexpression cells (right panel) and quantified by the Image J software. b EIF3H was knocked down by two different shRNA in KYSE150 and KYSE510 cells. The expression of Snail, EIF3H and some EMT markers, as described in (a), were analyzed by Immunoblotting in KYSE150-shEIF3H (left panel) and KYSE510-shEIF3H cells (right panel) and quantified by the Image J software. c and d Snail was knocked down in KYSE150-EIF3H overexpression or control cells (c) or overexpressed in KYSE150-shEIF3H#1 or shControl cells (d). The Snail and EIF3H expression were analyzed by immunoblotting (left panel). The migration and invasion ability of these cells was analyzed by the transwell assay (middle panel), and the migratory and invasive cells were counted (right panel). Scale bars, 500 μm

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