Fig. 2

Effect of hsa_circ_0002577 on EC cell proliferation. HEC-1-B cells were transfected with recombinant lentiviral vectors expressing hsa_circ_0002577 (Lv-circRNA) or the control vector (Lv-NC). Ishikawa cells were transfected with two shRNAs against hsa_circ_0002577 (sh-circRNA#1 and #2) or the negative control sequences. a The relative expression of hsa_circ_0002577 in transfected EC cells was detected using qRT-PCR. b CCK-8 assay was performed to evaluate cell proliferation in transfected EC cells at indicated time points (0, 24, 48, 72, and 96 h) post-transfection. The OD value at 450 nm was shown. c The number of colonies in transfected HEC-1-B and Ishikawa cells was measured 10 days after transfection. d TUNEL and DAPI staining was performed to detect apoptotic cell death in EC cells. The percentage of TUNEL-positive cells was calculated