Fig. 5From: CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathwayHsa_circ_0002577 regulates the expression of IGF1R via targeting miR-625-5p. a The target genes of miR-625-5p was predicted using Targetscan (www.targetscan.org), mirTarBase (http://mirtarbase.mbc.nctu.edu.tw/php/index.php), miRDB (www.mirdb.org), and miRwalk (http://zmf.umm.uni-heidelberg.de/apps/zmf/mirwalk/index.html). Ten genes were shown as potential target genes of miR-625-5p. b The luciferase vectors containing the 3′-UTR fragments of these genes (HOXB5, NFIX, IGF1R, MDM4, CCND1, SPSB1, SF3B3, SESN3, KIAA2013, and PRELP) were transfected into HEK-293 T cells. The luciferase activities were measured at 48 h post-transfection. c HEC-1-B cells were transfected with miR-625-5p inhibitor or control inhibitor (NC inh). Ishikawa cells were transfected with miR-625-5p mimics or control mimics (NC miR). The relative mRNA and expressions of HOXB5, SESN3, and IGF1R were analyzed using qRT-PCR. d The protein levels of these genes were measured using Western blot. e The putative binding site of IGF1R for miR-625-5p (IGF1R-WT) and the mutant sequence (IGF1R-MUT) were shown. f HEC-1-B cells were co-transfected with miR-625-5p inhibitor (or NC inh) and IGF1R-WT (or IGF1R-MUT). Ishikawa cells were co-transfected with miR-625-5p mimics (or NC miR) and IGF1R-WT (or IGF1R-MUT). The luciferase activity was determined 48 h after transfection by dual-luciferase reporter assay. g The mRNA expression of IGF1R in EC and normal tissues (n = 84). h The linear correlation coefficient was performed to estimate the correlation in the expression levels of hsa_circ_0002577 vs. IGF1R and miR-625-5p vs. miR-625-5p IGF1R. i HEC-1-B cells were co-transfected with Lv-circRNA (or Lv-NC) and miR-625-5p mimics (or NC miR). Ishikawa cells were co-transfected with sh-circRNA (or sh-NC) and miR-625-5p mimics (or NC miR). The protein expression level of IGF1R was measured by Western blot at 48 h post-transfectionBack to article page