Fig. 2
Genetically depletion of kiaa1522 gene impairs KrasG12D-induced lung adenocarcinoma and sensitizes tumors to cisplatin. a Schematic showing of adenovirus associated virus (AAV)-mediated kiaa1522 depletion in KrasG12D-induced lung. The Kras(LSL-G12D), LSL-Cas9 mice were intratracheal administrated by Cre- and control sgRNA or kiaa1522 sgRNAs-expressing-AAVs. Then, the mice were subjected to i.p. injections of vehicle/cisplatin. b The comparison of lung weights in different groups. t-test, **P < 0.01, ***P < 0.001. c-d Representative image of the lung after Bouin′s staining (c) and HE staining (d) in each group. e The comparison of tumor area in different groups. t-test, *P < 0.05, ****P < 0.0001. Bar = 2 mm. f Kaplan-Meier curves show the role of KIAA1522-depletion and/or effect of cisplatin on the outcome of lung cancer in mice. The survival rates were compared between each group. Log-rank test, *P < 0.05, **P < 0.01. g Immunohistochemical staining of cleaved-caspase 3 in the lung tumor samples from the indicated mice. t-test, ***P < 0.001. Bar = 100 μm