Fig. 5

KIAA1522 positively regulates TNFα-NFκB signaling in lung adenocarcinoma cells. a Immunoblotting assays of Phospho-NFκB p65 (Ser536) in the control and KIAA1522 down-regulated cells. b-c The indicated group of A549 cells (b) or 889 cells (c) were deprived of serum for 20 h, then treated with TNFα for 5 min. Then the cells were tested by Western blotting assay to detect the phosphorylation of p65, IKK and IκB proteins. d H1299 cells overexpressing Flag tagged KIAA1522 protein were cultured in serum-free condition for 20 h, then treated by TNFα and harvest at the indicated time point. The harvested cells were subjected to Western blotting assay. e Western blotting assays show the expression of TNFR2 and TNFR1 in KIAA1522 down-regulated A549 cells. f Western blotting assays show the expression of TNFR2 and TNFR1 in KIAA1522-overexpressed cells. g Immunohistochemical staining of TNFR2, phosphorylated IKKα/β and KIAA1522 in Kras-induced murine lung tumor samples with or without KIAA1522 depletion. Bar = 100 μm