Fig. 4

PSMB8-AS1 activity is directly and partially negatively regulated by miR-382-3p. a Bioinformatic prediction of the potential binding site of miR-382-3p and PSMB8-AS1. b Luciferase reporter assay analysis for the interaction with miR-382-3p and PSMB8-AS1. c Spearman’s rank correlation showing the negative correlation with miR-382-3p and PSMB8-AS1. d-e RT-qPCR assay indicating that PSMB8-AS1 was downregulated in the miR-382-3p overexpression PANC-1 and Mia paca2 cells. e-f CCK8 and clone overexpressing miR-382–3p partly inhibited the proliferation promoted by PSMB8-AS1. g-h Cell scratch and transwell assay showing that overexpressing miR-382–3p partly restrained the proliferation promoted by PSMB8-AS1