Fig. 2
TRIM59 binds and co-localizes with ABHD5. a. Network graph representation of interaction from the BioGRID for TRIM59. Users can select the ‘Network’ tab from the ‘Switch View’ menu to view interactions data when available. b. HEK-293 cells were transfected with HA-tagged TRIM59 and Myc-tagged ABHD5, lysates were prepared and immunoprecipitated (IP) with either anti-HA or anti-Myc, and the precipitates were evaluated using immunoblot (IB) analysis as noted. c-e. Immunohistochemical demonstration of the co-localization of TRIM59 and ABHD5 in THP-1 macrophages, BMDMs, and TAMs. Fluorescence images were counterstained with 4′6-diamidino-2-phenylindole (DAPI) for nucleus identification. Scale bars, 10 μm. f. THP-1 macrophages cells were transiently transfected with HA-tagged TRIM59 and Myc-tagged ABHD5. Rabbit anti-HA and Mouse anti-Myc antibody were used for the proximity ligation assay. Red dots present the interaction of TRIM59 with ABHD5. Scale bars, 20 μm