Fig. 4
Nicotine stimulates nuclear translocation of YAP1. a, Immunofluorescence assay of the localization of YAP in Panc-1 and BxPC3 cells upon nic (1.0 μM) or DMSO treatment for 24 h. Scale bars, 200 μm. b-c, Western blot assay of the expression of p-YAP(S127) in Panc-1 (b) and BxPC3 (c) cells with nicotine treatment at 0, 0.1, 1.0, 5.0 μM for 24 h. d-e, Western blot assays of the expression of YAP1 and p-YAP1 in the nuclear or cytoplasmic extracts in Panc-1 (d) and BxPC3 (e) cells with nic (1.0 μM) or DMSO treatment. Histone H3 and GAPDH were used as internal control for nuclear and cytoplasm, respectively. f-g, Western blot assay of the expression of MST1, p-MST1, LATS1 and p-LATS1 in Panc-1 and BxPC3 cells with nic (1.0 μM) or DMSO treatment. GAPDH was used as an internal reference. NE, nuclear extracts; CE, cytoplasmic extracts; Nic, nicotine. Data are shown as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001