Fig. 4
From: TM4SF1 promotes EMT and cancer stemness via the Wnt/β-catenin/SOX2 pathway in colorectal cancer
TM4SF1 drives the EMT and cancer stemness of CRC through Wnt/β-catenin signalling. a GSEA indicated several dysregulated Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. b Heat map of the indicated target genes in TM4SF1-silenced and control cells. The target genes of Wnt/β-catenin signalling were decreased in TM4SF1-silenced cells. c TM4SF1 knockdown reduced the levels of total β-catenin and the nuclear translocation of β-catenin. d, e LiCl restored the expression of Wnt/β-catenin target genes, EMT and stemness markers in TM4SF1-deficient cells. f-g Activation of β-catenin rescued the EMT phenotype and diminished the TM4SF1 deficiency-mediated inhibitory effect on cell migration and invasion. h LiCl promoted tumour sphere formation in TM4SF1-deficient cells. The tumourspheres were counted, and the percentage of tumourspheres with diameters 50–100 μm, 100–150 μm, and > 150 μm was calculated. *P < 0.05