Fig. 6

c-Myc directly binds the SOX2 promoter and regulates SOX2 expression. a LiCl significantly upregulated the expression of SOX2 and c-Myc in TM4SF1 knockdown CRC cells. b SOX2 deficiency significantly downregulated the expression of SOX2 but not the expression of c-Myc in the LiCl-treated cells. c c-Myc knockdown decreased the expression of SOX2. d c-Myc overexpression upregulated the expression of SOX2. e Promoter region of the SOX2 gene with a putative c-Myc binding site. f ChIP assays assessing the binding of c-Myc to the SOX2 promoter. Normal rabbit IgG and anti-c-Myc antibody were used to precipitate chromatin DNA fragments as indicated. g, h SOX2 knockdown suppressed migration and tumour sphere formation in c-Myc-overexpressing cells. i SOX2 deficiency decreased the expression of N-cadherin, CD133, and CD44 and upregulated the expression of E-cadherin in c-Myc-overexpressing cells. *P < 0.05