Fig. 4
From: Autophagic flux inhibition enhances cytotoxicity of the receptor tyrosine kinase inhibitor ponatinib
Combo therapy interferes with autophagy flux in human neuroblastoma cells. a-c Representative co-immunostaining of LC3 (green) and LAMP-2 (red) proteins are shown for the neuroblastoma cells treated according to the indicated schemes. Histograms show the percentage (%) of double-positive (yellow puncta) staining (autolysosomes). DAPI was used to counterstain nuclei. Scale bar, 100 μm. *p < 0.05; **p < 0.01; n.s. – not significant