Fig. 6

Histopathological examination of post-therapy neuroblastoma tumors confirms the efficacy of combination treatment in vivo. a Hematoxylin & Eosin (H&E) of tumor tissues and immunohistochemistry staining of CD56 proteins were carried out. Red arrows indicate positive cellular labeling. Scale bar, 150 μm. b Immunohistochemistry staining of Ki67, c active CASPASE 3, and d TUNEL assay for the detection of proliferating and apoptotic cells, respectively. The percentage (%) of positive events is reported in the histograms on the right. Scale bar, 150 μm. e Immunohistochemistry staining of LC3-positive cells in resected post-therapy tumors. Red arrows indicate positive cellular labeling. Scale bar, 150 μm. The percentage (%) of LC3-positive cells is reported in the histogram on the right. *p < 0.05, **p < 0.01, ***p < 0.001