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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Aptamer targeted therapy potentiates immune checkpoint blockade in triple-negative breast cancer

Fig. 2

Gint4.T aptamer specifically binds to and inhibits migration of PDGFRβ-positive murine cells. a Binding curve of 5′-biotinylated PDGFRβ Gint4.T aptamer to 4 T1 cells for calculation of the apparent Kd of aptamer-cell interaction. The background binding value for 5′-biotinylated Scr sequence was subtracted from each data point. Data shown are mean ± SD (n = 3). b Representative confocal images of 4 T1, NIH3T3 and BT-474 cells incubated with FAM-Gint4.T or FAM-Scr, as indicated. FAM-aptamers and nuclei are visualized in green and blue, respectively. All digital images were captured at the same setting to allow for direct comparison of staining patterns. Magnification 63 ×, scale bar = 20 μm. c Left, 4 T1 and NIH3T3 cell migration toward PDGF-BB was analyzed by transwell migration assay in the presence of Gint4.T or Scr. Photographs of a representative experiment are shown. Magnification 5 ×, scale bar = 500 μm. Right, data were presented as percentage of migrated cells in the presence of Gint4.T with respect to cells treated with Scr. Bars depict mean ± SD (n = 3). *** P < 0.001, ** P < 0.01 relative to Scr; unpaired t-test

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