Fig. 3

Effects of combinatorial treatments on murine 4 T1 cells in the absence or presence of lymphocytes. a Cell growth inhibition of 4 T1 cells, untreated or treated with the anti-mPD-L1 mAb or the PDGFRβ aptamer, used alone (dark grey bars) or in combination (black bars), for 96 h at 37 °C at the indicated concentrations. Untreated cells (white bars) or cells treated with an unrelated IgG or Scr (light grey bars) were used as negative controls. Data were expressed as percentage of viable treated cells with respect to untreated cells. b-e 4 T1 cells were co-cultured with mouse lymphocytes and treated for 24 h, as indicated. b Cell counts were expressed as percentage of viable treated cells with respect to untreated co-cultured cells. c Cell lysis was measured by LDH release in the medium after incubation with the indicated compounds. d IL-2 and e IFN-γ secretion levels were measured by ELISA on supernatants of cells treated as indicated. a-e Error bars depict mean ± SD (n = 3). ^#### P < 0.0001, ^## P < 0.01 relative to IgG; ^°°°° P < 0.0001, ^°° P < 0.01, ° P < 0.05 relative to Scr; ^**** P < 0.0001, ^*** P < 0.001, ^** P < 0.01, ^* P < 0.05; one-way ANOVA followed by Tukey’s multiple comparison test