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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Long non-coding RNA LPP-AS2 promotes glioma tumorigenesis via miR-7-5p/EGFR/PI3K/AKT/c-MYC feedback loop

Fig. 1

RNA-seq analysis reveals that LPP-AS2 is significantly upregulated in glioblastoma tissues and transcriptionally regulated by c-MYC. a Flow diagram describes the steps for identifying and validating lncRNAs in glioblastoma. b The KEGG pathway analysis of differentially expressed lncRNAs. c Heatmaps of top 50 lncRNAs that were differentially expressed between glioblastoma and normal brain tissues. Upregulated lncRNAs are shown in red and downregulated lncRNAs are shown in blue. d Relative expression of LPP-AS2 was significantly elevated in (12 Grade I, 15 Grade II, 30 Grade III and 49 Grade IV) glioma tissues compared with 23 normal brain tissues. e Kaplan-Meier analysis of overall survival in glioma patients with low (n = 167) and high (n = 168) level of LPP-AS2. P value was calculated by Mantel-Cox log rank test. f Pulldown of LPP-AS2 in Chromatin immunoprecipitation assay with c-MYC. Results of western blots suggesting c-MYC could effective pulldown of LPP-AS2, and β-actin as the negative control. g Illustration of c-MYC bound to the promoter regions of LPP-AS2. Transcription start site (TSS) was designated as + 1. The putative binding sites were listed. h C-MYC significantly enhanced the fold enrichment of LPP-AS2 probe compared with IgG in U251 and SHG44 cells. i Relative level of LPP-AS2 in the nuclear and cytoplasmic fractions of U251 and SHG44 cells. j Relative expression of LPP-AS2 in seven glioma cell lines and HEB by RT-qPCR. Error bars, s.e.m. from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (Student’s t test)

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