Fig. 2
LPP-AS2 promotes glioblastoma progression in vitro. a Relative expression of LPP-AS2 in U251 and SHG44 cells transfected with siRNAs/overexpression plasmids. b CCK-8 assays in U251 and SHG44 cells with silencing/overexpression of LPP-AS2. c Colony formation assays in U251 and SHG44 with silencing/overexpression of LPP-AS2. d Transwell assays in U251 and SHG44 cells with silencing/overexpression of LPP-AS2. Representative staining images are presented (bar = 50 μm). e Wound healing assays of U251 and SHG44 cells with silencing/overexpression of LPP-AS2. f TUNEL assays in U251 and SHG44 cells with silencing/overexpression of LPP-AS2 (bar = 200 μm). g Flow cytometry analysis in U251 and SHG44 cells with silencing/overexpression of LPP-AS2. Data represent the mean ± s.e.m. of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (Student’s t test)