Fig. 5

LPP-AS2 regulates glioblastoma progression by directly bind with miR-7-5p. a Relative expression of miRNAs align to LPP-AS2. b Knockdown of LPP-AS2 significantly upregulated the expression of miR-7-5p in U251 and SHG44 cells. c The Pearson correlation between LPP-AS2 level and miR-7-5p level was measured in the same set of glioblastoma tissues. The ΔCt values (normalized to GAPDH) were subjected to Pearson correlation analysis (R = -0.7504, P < 0.0001). d Ago2 protein immunoprecipitation was used to detect the fold enrichment of LPP-AS2 in U251 and SHG44 cells. e Schematic representation of the potential binding sites between LPP-AS2 and miR-7-5p. Luciferase report activity of constructed plasmids (LPP-AS2-WT or LPP-AS2-MUT) in 293 T cells co-transfected with miR-7-5p mimics or miR-NC mimics. f Lnc_LPP-AS2 pulldown was used to measure the fold enrichment of LPP-AS2 and miR-7-5p in U251 and SHG44 cells. g Enrichment of LPP-AS2 in U251 and SHG44 cells after treated with biotinylated miR-7-5p. h RT-qPCR analysis of miR-7-5p expression in U251 and SHG44 cells transfected with miR-7-5p inhibitor alone or co-transfection with siRNAs of LPP-AS2. i CCK8 assays in U251 and SHG44 cell treated with miR-7-5p inhibitor alone or co-transfection with siRNAs of LPP-AS2. j Histogram of invasion assays in U251 and SHG44 cells treated with miR-7-5p inhibitor alone or co-transfection with siRNAs of LPP-AS2. k Histogram of colony formation assays in glioma cells treated with miR-7-5p inhibitor alone or co-transfection with siRNAs of LPP-AS2. l Histogram of flow cytometry assays in U251 and SHG44 cells treated with miR-7-5p inhibitor alone or co-transfection with siRNAs of LPP-AS2. Data represent the mean ± s.e.m. of three independent experiments. ns (no significance); *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ^#P < 0.05; ^##P < 0.01; ^###P < 0.001; ^####P < 0.0001 (Student’s t test)