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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Long non-coding RNA LPP-AS2 promotes glioma tumorigenesis via miR-7-5p/EGFR/PI3K/AKT/c-MYC feedback loop

Fig. 6

LPP-AS2 decoys miR-7-5p to regulate the expression of EGFR and its downstream PI3K/AKT/c-MYC signaling pathway. a A total of 62 targeted mRNAs of miR-7-5p from four online databases (miRDB, miRTarBase, miRWalk, TargetScan). b Schematic representation of the potential binding sites between miR-7-5p and EGFR. Luciferase reporter activity of constructed plasmids (EGFR-WT or EGFR-MUT) in 293 T cells co-transfected with miR-7-5p mimics or miR-NC mimics. c Relative expression of EGFR in U251 and SHG44 cells transfected with miR-7-5p mimics or miR-7-5p inhibitor. d Protein level of EGFR in U251 and SHG44 cells transfected with miR-7-5p mimics or miR-7-5p inhibitor. e The Pearson correlation between EGFR level and miR-7-5p level was measured in the same set of glioblastoma tissues. The ΔCt values (normalized to GAPDH) were subjected to Pearson correlation analysis (R = -0.7493, P < 0.0001). f Western blotting analysis of EGFR, PI3K, AKT, p-PI3K, p-AKT, c-MYC in U251 and SHG44 cells treated with miR-7-5p inhibitor alone or co-transfection with siRNAs of LPP-AS2. g Histogram of EGFR protein levels in U251 and SHG44 cells treated with miR-7-5p inhibitor alone or co-transfection with siRNAs of LPP-AS2. h EGFR mRNA expression in U251 and SHG44 cells treated with miR-7-5p inhibitor alone or co-transfection with siRNAs of LPP-AS2. i, j Ratio of p-PI3K/PI3K and p-AKT/AKT in U251 and SHG44 cells. k Western blotting analysis of EGFR, PI3K, p-PI3K, AKT, p-AKT and c-MYC in U251 and SHG44 cells treated with pEGFP-C1/EGFR plasmids alone or co-transfection with si-LPP-AS2–1. l Changes of p-PI3K/PI3K and p-AKT/AKT in U251 and SHG44 cells treated with pEGFP-C1/EGFR plasmids alone or co-transfection of si-LPP-AS2–1. Data represent the mean ± s.e.m. of three independent experiments. ns (no significance); *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ##P < 0.01; ###P < 0.001; ####P < 0.0001 (Student’s t test)

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