Fig. 3

NUCKS restoration rescues the cell proliferation and cell-cycle arrest of NUCKS-silenced gastric cancer cells. a When NUCKS overexpressed in shNUCKS#1 knockdown cells, NUCKS expression was detected using qRT-PCR. The NUCKS-silenced cells were manufactured using the shNUCKS#1 plasmid in all follow-up experiments. b NUCKS restoration rescued the proliferation of HGC-27 and SGC-7901 cells. MTT assay was performed to examine the effect of NUCKS overexpressed on cell viability. c BrdU assays were performed after NUCKS overexpressed. Representative images show immunofluorescence and the quantification of BrdU-positive cells (Scale bars, 20 μm). Data were analyzed using 2-tailed Student t tests (**P < 0.01, ***P < 0.001). d Western blot assay was performed to detect the expression of S-phase cell cycle regulatory proteins in NUCKS-overexpressed cells