Fig. 5

NUCKS silencing induces gastric cancer cell autophagy via the mTOR-Beclin1 signaling pathway. a, b GSEA shows significant enrichment of gene sets involved in autophagy process (left) and mTOR signal pathway (right). NOM = nominal, FDR = false discovery rate. c The quantitative analysis of mTOR expression in after NUCKS knockdown and restoration (**P < 0.01, ***P < 0.001). d The protein expression of mTOR pathway in NUCKS-knockdown and overexpression cells were measured by western blot assay. e Western blot analysis of mTOR, Beclin1 and LC3B expression when silencing Beclin1 on NUCKS-knockdown cells. f Western blot analysis of the mTOR signaling pathway after 10 nM Rapamycin treatment in NUCKS-restored cells