Fig. 6

NUCKS promotes colony formation in vitro and gastric cancer cell tumor formation in vivo. a Soft agar assays were performed after NUCKS knockdown and restoration in the HGC-27 and SGC-7901 cell lines (Scale bars, 500 μm). The colony numbers were also quantified (**P < 0.01, ***P < 0.001). b, c Growth monitor (left), weights and photographs (right) of the indicated xenograft tumors. Data were analyzed using two-tailed Student’s t-tests (*P < 0.05, **P < 0.01, ***P < 0.001). d IHC analysis of the NUCKS or Ki-67 expression in the indicated xenograft tumors (Scale bars, 50 μm)