Fig. 4

Downregulation of hsa_circ_0001944 suppressed BC invasion both in vitro and in vivo. a and b Cell migration was assessed in both T24 and UMUC3 cells using transwell assays. Data are presented as mean ± SD; ^***P < 0.001 vs NC. c and d Wound healing assays show the invasion ability of both T24 and UMUC3 cells. Data are presented as mean ± SD; ^***P < 0.001 vs NC. e Live imaging showing the effects of hsa_circ_0001944 on the metastasis of T24 cells 30 d after intravenous tail injection. f Immunohistochemistry showing the percentage of Ki-67-positive cells. g-i RT-qPCR detection show the expression of hsa_circ_0001944 (G), miR-548 (H) and PROK2 (I) in tumor tissues. Data are presented as mean ± SD; ^***P < 0.001 vs. NC