Fig. 2

VEGFA derived from hypoxic PMCs facilitates GC cell adhesion and migration via VEGFR1. a The effect of exogenous VEGFA on cell migration was observed in the presence of Apatinib or Bevacizumab. Representative photographs of migratory cells are shown. Scale bar represents 100 μm. Error bars represent SD of the mean. *P < 0.05. b Western blot analysis of VEGFR1 protein levels in various GC cells. c Survival analysis for different VEGFR1 expression levels using the KM-Plotter database of GC. d MGC-803 sgRNA-VEGFR1 and sgRNA-NC cells were intraperitoneally inoculated. The metastatic nodules (green arrows) were analyzed after 20 days (N = 5 per group). Representative data are shown. ****P < 0.0001. e and f MGC-803 cells were exposed to CM from hypoxic PMCs or exogenous VEGFA, synchronously with knockout of VEGFR1 using sgRNA-FLT1. Representative photographs of adherent and migratory cells are shown. Scale bar represents 100 μm. Error bars represent SD of the mean. *P < 0.05. **P < 0.01. ***P < 0.001