Fig. 3
VEGFA derived from hypoxic PMCs promotes the expression of integrin α5/fibronectin via VEGFR1. a and b Gene Set Enrichment Analysis (GSEA) indicating that “FOCAL_ADHESION” is significantly associated with VEGFR1. KEGG pathway enrichment analysis identified the KEGG_FOCAL_ADHESION pathway in CM-treated MGC-803 cells by gene array analysis. c Three genes were identified that overlapped between the gene signature of GSEA enrichment analysis and the KEGG pathway enrichment analysis. d Survival analysis for different integrin α5 and fibronectin expressions using the KM-Plotter database of GC. The Oncomine database showed the differential expression of integrin α5/fibronectin in gastric carcinoma tissues and gastric mucosal tissues. e Immunoblot analysis of integrin α5 and fibronectin after cultivation of GC cells with CM from hypoxic cells. f Immunoblot analysis of p-JNK and p-ERK in GC cells at the indicated time points following the addition of 100 ng/mL VEGFA. g VEGFR1 was knocked out with sg-RNA and the expression of integrin α5 and fibronectin was detected after cultivation of GC cells with CM from hypoxic cells. h VEGFR1 was knocked out with sg-RNA and the expression of integrin α5, fibronectin, p-JNK and p-ERK was determined after exogenous VEGFA application