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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Hypoxia-autophagy axis induces VEGFA by peritoneal mesothelial cells to promote gastric cancer peritoneal metastasis through an integrin α5-fibronectin pathway

Fig. 4

VEGFA derived from hypoxic PMCs promotes adhesion and migration through the expression of integrin α5/fibronectin. a The indicated cells were treated with exogenous VEGFA and immunoprecipitation was performed with an integrin α5 antibody, followed by detection of integrin α5 or fibronectin by immunoblot analysis. b Immunoblotting of integrin α5 was performed in the indicated cells following transfection with si-integrin α5-170A or si-integrin α5-170B for 24 h. c and d MGC-803 and SGC-7901 cells were exposed to CM from hypoxic PMCs, synchronously with knockdown of integrin α5. Representative photographs of adherent and migratory cells are shown. Scale bar represents 100 μm. Bars represent SD of the mean. *P < 0.05. **P < 0.01. ***P < 0.001

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Journal of Experimental & Clinical Cancer Research

ISSN: 1756-9966

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