Fig. 1

miR-195-5p and miR-497-5p were poorly expressed in CCSCs. a, Heat map of differentially expressed miRNAs between tumor samples and normal samples from the GSE108183 dataset. The x-axis represents the sample number, while the y-axis represents the name of the miRNA, the left dendrogram represents the clustering of miR expression, and the upper right histogram indicates the color gradation. b, Sequences for miR-497-5p and miR-195-5p. c, The expression of miR-195-5p and miR-497-5p in colon cancer tissues (n = 35) and adjacent tissues (n = 35) as detected by RT-qPCR. d, The expression of miR-195-5p and miR-497-5p in colon cancer cell lines (LoVo, SW620, SW1116, SW480 and HCT-116) and immortalized colon epithelial cell lines (NCM460) as detected by RT-qPCR. e, The morphology of colon cancer cells SW620 and LoVo and their corresponding CCSCs (× 200) as observed under the optical microscope. f, The expression of stem cell markers (Nanog, Oct-4, and SOX-2) in colon cancer cells and CCSCs, as detected by Western blot analysis. g, The expression of miR-195-5p and miR-497-5p in CCSCs and colon cancer cells were as detected by RT-qPCR. Measurement data were expressed as mean ± s.d. Data comparison between colon cancer tissues and adjacent tissues were compared using paired t-test. Data among multiple groups were compared using one-way analysis of variance and further analyzed with Tukey’s post-hoc test. Cell experiment was repeated three times. * p < 0.05 vs. control