Fig. 5

NF-κB activation downregulates miR-195-5p/497–5p and thereby promotes tumorigenesis and stem-like properties of CCSCs in vivo. a & b, CCSCs were treated with overexpressed miR-195-5p/497–5p and then subcutaneously injected into nude mice to establish a subcutaneous xenograft tumor model. At the same time, mice were injected with LPS or PBS twice a week until the day before the end of the experiment. The volume and weight of xenografted tumors were observed and recorded (n = 6 for each group). c, RNA was extracted from tumor tissues and the expression of miR-195-5p and miR-497-5p was detected by RT-qPCR. d, The protein expression of p65, extent of p65 phosphorylation, CD133, Nanog, Oct-4, Sox-2, and EpCAM in tumor tissues as detected by Western blot analysis. e, The protein expression of apoptosis-related proteins (Bcl-2 and Bax) in tumor tissues as detected by Western blot analysis. (1, miR-NC + PBS; 2, miR-NC + LPS; 3, miR-497-5p/195-5p agomir + PBS; 4, miR-497-5p/195-5p agomir + LPS). Measurement data were expressed as mean ± s.d. Data between two groups were compared using unpaired t-test. Data among multiple groups were compared using one-way analysis of variance and then analyzed with Tukey’s post-hoc test. Repeated measures analysis of variance was used for comparing data among multiple groups at different time points, followed by Bonferroni post-hoc test. Cell experiment was repeated three times. * p < 0.05 vs. miR-NC + PBS. # p < 0.05 vs. miR-497-5p/195-5p agomir + PBS